Fmoc-β-Ala-Asp(OtBu)-OH Acidic amino acid (Asp) introduction SPPS

Used in Solid-Phase Peptide Synthesis:

• Incorporation of β-Ala–Asp fragment

• Reduces coupling steps

• Improves synthesis efficiency

Asp provides:

• Negative charge (after deprotection)

• Improves peptide solubility

• Enables electrostatic interactions

• Prevents side reactions (e.g., cyclization)

• Ensures clean peptide elongation

• Removed under acidic conditions (TFA)

• Adds flexibility

• Reduces steric hindrance

• Optimizes peptide conformation